Plakophilin 1 Antibody
Recognizes a protein of 75kDa, identified as Plakophilin 1 (PKP1) . Plakophilins 1, 2, 3 and 4 (PKP1-4) influence development and participate in linking cadherins to cytoskeletal intermediate filaments. PKP1-4 contain arm-repeat (armadillo) domains and localize to nuclei and cell desmosomes (cell-cell junctions found in suprabasal layers of stratifying epithelia that undergo mechanical stress) . PKP2 is important for desmosome assembly and is an essential morphogenic factor and architectural component of the heart.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL
UniProt
Q13835
Host
Mouse
Reactivity
Human, Mouse
Immunogen
Clonality
Monoclonal
Isotype
IgG1 k
Clone
10B2
Applications
FACS, IF, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Plakophilin 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Plakophilin 1 antibody is available for research use only.
Storage Conditions
Store the Plakophilin 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Plakophilin 1 antibody should be determined by the researcher.
Location
Cell surface, nuclear
Image Legend
Western blot testing of human 1) U87, 2) HeLa and 3) A431 cell lysate with Plakophilin 1 antibody (clone 10B2) . Expected molecular weight: 75-83 kDa.
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