TIMP2 Antibody

It recognizes a protein of 21kDa, identified as tissue inhibitor of metalloproteinases 2 (TIMP2) . It is closely related to TIMP1 and shows the highest binding affinity to both the latent (pro) and active forms of 72kDa Type IV collagenase (also known as MMP2 or gelatinase A) . It also has affinity for the active form of 92kDa Type IV collagenase (also known as MMP9 or gelatinase B) . TIMP2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P16035

Host

Mouse

Reactivity

Human

Immunogen

An amino acid sequence from the N-terminal region of the human protein was used as the immunogen for this TIMP2 antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

SPM356

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the TIMP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This TIMP2 antibody is available for research use only.

Storage Conditions

Store the TIMP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TIMP2 antibody to be titered up or down for optimal performance.

Location

Cytoplasmic, cell surface, secreted

Image Legend

IHC testing of FFPE human placenta with TIMP2 antibody (clone SPM356) . Required HIER: requires boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.