EPO Antibody / Erythropoietin

Erythropoietin / EPO is a secreted, glycosylated cytokine hormone composed of four alpha helical bundles. It is the primary factor responsible for regulating erythropoiesis during steady-state conditions and in response to blood loss and hemorrhage in the adult organism. Erythropoietin is synthesized by the kidney and stimulates the proliferation and maturation of bone marrow erythroid precursor cells. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: 2-4 µg/mL (order BSA/azide-free format), Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

UniProt

P01588

Host

Mouse

Reactivity

Human

Immunogen

A human recombinant partial protein was used as the immunogen for the EPO antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

EPO/1368

Applications

ELISA, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the EPO antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This EPO antibody is available for research use only.

Storage Conditions

Store the EPO antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the EPO antibody may be required for optimal performance.1. Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min.