P63 Antibody
P63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands; however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S) . p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90%. Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80%. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA: order BSA/sodium azide-free format for coating, Western blot: 1-2 µg/mL
UniProt
Q9H3D4
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 3-106 were used as the immunogen for this p63 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
TP63/1786
Applications
ELISA, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the p63 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This p63 antibody is available for research use only.
Storage Conditions
Store the p63 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the p63 antibody for each application should be determined by the researcher.
Location
Nuclear
Image Legend
Western blot testing of human MCF-7, T-47D and MDA-MB-453 lysate with p63 antibody (clone TP63/1786) . Expected molecular weight: 63-77 kDa.
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