P63 Antibody

P63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands; however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S) . p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90%. Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80%. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q9H3D4

Host

Rabbit

Reactivity

Human

Immunogen

Recombinant partial human p63 protein was used as the immunogen for this p63 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the p63 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This p63 antibody is available for research use only.

Storage Conditions

Store the p63 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The optimal dilution of the p63 antibody for each application should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of FFPE human prostate cancer tissue with p63 antibody. Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.