Filaggrin Antibody

Filaggrin is essential for the regulation of epidermal homeostasis. Within the stratum corneum, filaggrin monomers can become incorporated into the lipid envelope, which is responsible for the skin barrier function. Alternatively, these proteins can interact with keratin intermediate filaments. Filaggrin undergoes further processing in the upper stratum corneum to release free amino acids that assist in water retention. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P20930

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human FLG protein was used as the immunogen for this Filaggrin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

SPM181

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Filaggrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Filaggrin antibody is available for research use only.

Storage Conditions

Store the Filaggrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Filaggrin antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human skin with Filaggrin antibody (clone SPM181) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.