NKX2.2 Antibody
Expression of NKX2.2 has been found in neuroendocrine tumors of the gut, making it a potential marker for the study of gastrointestinal neuroendocrine tumors. More recently, NKX2.2 protein was identified as a target of EWS-FLI-1, the fusion protein specific to Ewing sarcoma, and was shown to be differentially upregulated in Ewing sarcoma on the basis of array-based gene expression analysis. It acts as a valuable marker for Ewing sarcoma, with a sensitivity of 93% and a specificity of 89%, and aids in the differential diagnosis of small round cell tumors.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
O95096
Host
Mouse
Reactivity
Human
Immunogen
A human partial recombinant protein corresponding to amino acids 1-119 was used as the immunogen for this NKX2.2 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
NX2/1524
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the NKX2.2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This NKX2.2 antibody is available for research use only.
Storage Conditions
Store the NKX2.2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the NKX2.2 antibody may be required for optimal performance.
Location
Nuclear
Image Legend
IHC testing of FFPE Ewings sarcoma with NKX2.2 antibody (clone NX2/1524) . HIER: steam sections in 10mM citrate buffer, pH 6, for 10-20 min.
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