Villin Antibody / VIL1
Recognizes a protein of ~93kDa which is identified as Villin (VIL1) . It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL for 60 min at RT, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT
UniProt
P09327
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
VIL1/1314
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the Villin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Villin antibody is available for research use only.
Storage Conditions
Store the Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titration of the Villin antibody may be required for optimal performance.
Location
Cytoplasmic and cell surface
Image Legend
Frequently Asked Questions
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