Villin Antibody / VIL1

Recognizes a protein of ~93kDa which is identified as Villin (VIL1) . It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, Villin is predominantly expressed in tumors of colorectal origin. Antibody to Villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL for 60 min at RT, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

UniProt

P09327

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 179-311 from the human protein were used as the immunogen for this Villin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

VIL1/1314

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Villin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Villin antibody is available for research use only.

Storage Conditions

Store the Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titration of the Villin antibody may be required for optimal performance.

Location

Cytoplasmic and cell surface

Image Legend

IHC testing of FFPE human rectum tissue with Villin antibody (clone VIL1/1314) . Required HIER: boil tissue sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling at RT for 20 min.