Ferritin Light Chain Antibody

Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III) . Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA: order BSA/sodium azide-free format for coating, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL

UniProt

P02792

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 38-165 of human FTL were used as the immunogen for this Ferritin Light Chain antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

FTL/1386

Applications

ELISA, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the Ferritin Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Ferritin Light Chain antibody is available for research use only.

Storage Conditions

Store the Ferritin Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Ferritin Light Chain antibody to be titered up or down for optimal performance.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human pancreas with Ferritin Light Chain antibody (clone FTL/1386) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.