FTL Antibody / Biotin Conjugate

Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III) . Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order Ab without BSA for coating), Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

UniProt

P02792

Host

Mouse

Reactivity

Human

Immunogen

A portion of amino acids 38-165 from the human protein was used as the immunogen for the FTL antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

FTL/1386

Applications

ELISA, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Biotin Conjugate

Buffer

0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

Reconstitution

Store the FTL antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

Limitations

This FTL antibody is available for research use only.

Storage Conditions

Store the FTL antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

Formulation

0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

Applications Notes

Optimal dilution of the FTL antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC staining of FFPE human kidney with biotin-conjugated FTL antibody (clone FTL/1386) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.