Galectin 13 Antibody / LGALS13

It recognizes a 32kDa protein, which is identified as homodimer of LGALS13/Galectin 13 (also known as PP13) . Galectins are a family of soluble β -galactoside-binding lectins that modulate cell-to-cell adhesion and cell-to-extracellular matrix (ECM) interactions and play a role in tumor progression, pre-mRNA splicing and apoptosis. One such member, Galectin 13, is a 139 amino acid protein that contains one galectin domain. Possessing lysophospholipase activity, Galectin-13 exists as a disulfide-linked homodimer. Galectin 13 is suggested to have a developmental role in the placenta and may display immunobiological activity in feto-maternal blood-spaces. Screening for Galectin 13 in maternal serum during the first trimester of pregnancy may serve as a diagnostic marker to predict preterm pre-eclampsia.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

Q9UHV8

Host

Mouse

Reactivity

Human

Immunogen

A human partial recombinant protein corresponding to amino acids 23-134 was used as the immunogen for the Galectin 13 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

PP13/1164

Applications

ELISA, FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Galectin 13 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Galectin 13 antibody is available for research use only.

Storage Conditions

Store the Galectin 13 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the Galectin 13 antibody may be required for optimal performance.

Location

Cytoplasmic, cell surface

Image Legend

IHC testing of FFPE human placenta with Galectin 13 antibody (clone PP13/1164) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9 or 10mM Citrate buffer, pH 6, for 10-20.