DOG 1 Antibody

DOG 1 is expressed in the human gastrointestinal epithelium and is highly expressed in the gastrointestinal interstitial cells of Cajal, where it plays an important role in epithelial chloride secretion mediating intestinal motility. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q5XXA6

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human protein corresponding to amino acids 2-101 of human DOG1/ANO1 was used as the immunogen for this DOG 1 antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

DG1/1484

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the DOG 1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This DOG 1 antibody is available for research use only.

Storage Conditions

Store the DOG 1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the DOG 1 antibody to be titered up or down for optimal performance.

Location

Cell Surface and Cytoplasmic

Image Legend

IHC testing of FFPE human gastrointestinal stromal tumor (GIST) with DOG 1 antibody (DG1/1484) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.