CDH3 Antibody / P-Cadherin

Recognizes a protein of 116kDa, identified as P-Cadherin, or Cadherin 3 or CDH3. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. This gene is located in a six-cadherin cluster in a region on the long arm of chromosome 16 that is involved in loss of heterozygosity events in breast and prostate cancer. In addition, aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in this gene have been associated with congenital hypotrichosis with juvenile macular dystrophy.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL

UniProt

P22223

Host

Mouse

Reactivity

Human

Immunogen

A human full length recombinant protein was used as the immunogen for this P-Cadherin / CDH3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

12H6

Applications

FACS, IF, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CDH3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CDH3 antibody is available for research use only.

Storage Conditions

Store the CDH3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CDH3 antibody to be titered up or down for optimal performance.

Location

Cell surface, cytoplasmic

Image Legend

Western blot testing of human A431 cell lysate with P-Cadherin / CDH3 antibody (clone 12H6) . Expected molecular weight: ~91 kDa (unmodified), 100~130 kDa (glycosylated) .