Glycophorin A Antibody

Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus. Is a receptor for Plasmodium falciparum erythrocyte-binding antigen 175 (EBA-175) ; binding of EBA-175 is dependent on sialic acid residues of the O-linked glycans. Appears to be a receptor for Hepatitis A virus (HAV) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL for 30 min at RT

UniProt

P02724

Host

Mouse

Reactivity

Human

Immunogen

Formalin fixed membrane from a Hairy cell leukemia was used as the immunogen for the Glycophorin A antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

JC 159

Applications

FACS, IF, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Glycophorin A antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Glycophorin A antibody is available for research use only.

Storage Conditions

Store the Glycophorin A antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the Glycophorin A antibody should be determined by the researcher.

Location

Cytoplasmic, membranous

Image Legend

IHC testing of FFPE human tonsil tissue with Glycophorin A antibody (clone JC 159) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.