Histone H1 Antibody
Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt]
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL
UniProt
P07305
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant full-length human protein was used as the immunogen for the Histone H1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
FACS, IF, IHC-P, WB
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Histone H1 antibody is available for research use only.
Storage Conditions
Store the Histone H1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Histone H1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Immunofluorescent staining of PFA-fixed human HeLa cells with Histone H1 antibody (green) and Phalloidin (red) .
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