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Histone H1 Antibody

Histones H1 are necessary for the condensation of nucleosome chains into higher-order structures. The H1F0 histones are found in cells that are in terminal stages of differentiation or that have low rates of cell division. [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL

UniProt

P07305

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full-length human protein was used as the immunogen for the Histone H1 antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

FACS, IF, IHC-P, WB

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Histone H1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Histone H1 antibody is available for research use only.

Storage Conditions

Store the Histone H1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Histone H1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

Immunofluorescent staining of PFA-fixed human HeLa cells with Histone H1 antibody (green) and Phalloidin (red) .

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