Cytokeratin 8/18 Antibody

Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18) . This mAb recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 2-5ul/10^6 cells, Immunofluorescence: 1:100-1:200, Immunohistochemistry (FFPE) : 1:50-1:100 for 30 min at RT

UniProt

P05787, P05783

Host

Mouse

Reactivity

Human

Immunogen

A cytoskeleton preparation from human breast cancer MCF-7 cells was used as the immunogen for the Cytokeratin 8/18 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

5D3

Applications

FACS, IF, IHC-P

Format

Bioreactor concentrate

Buffer

Bioreactor concentrate with 0.05% sodium azide

Reconstitution

Store the Cytokeratin 8/18 antibody at 2-8oC.

Limitations

This Cytokeratin 8/18 antibody is available for research use only.

Storage Conditions

Store the Cytokeratin 8/18 antibody at 2-8°C.

Formulation

Bioreactor concentrate with 0.05% sodium azide

Applications Notes

Optimal dilution of the Cytokeratin 8/18 antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC analysis of FFPE human colon carcinoma with Cytokeratin 8/18 antibody (clone 5D3) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.