Lewis y Antibody
This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-) . Lewis y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
Not Applicable
Host
Mouse
Reactivity
Human
Immunogen
Live Ls174T cells (human colon carcinoma) were used as the immunogen for the Lewis y antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
A70-A/A9
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Lewis y antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Lewis y antibody is available for research use only.
Storage Conditions
Store the Lewis y antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Lewis y antibody should be determined by the researcher.
Location
Cell membrane, cytoplasmic and extracellular
Image Legend
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