Connexin 32 Antibody / GJB1
This Ab recognizes a protein of 27-32kDa, identified as Connexin 32. The connexin family of proteins forms hexameric complexes called connexons that facilitate movement of low molecular weight proteins between cells via gap junctions. Connexin proteins share a common topology of four transmembrane α -helical domains, two extracellular loops, a cytoplasmic loop and cytoplasmic N- and C-termini. Many of the key functional differences arise from specific amino-acid substitutions in the most highly conserved domains, the transmembrane and extracellular regions. Each of the approximately 20-connexin isoforms produces channels with distinct permeability and electrical and chemical sensitivities; therefore, one connexin usually cannot fully substitute for another.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunofluorescence: 1-2 µg/mL, Flow cytometry: 0.5-1ug/10^6 cells, Western blot: 0.5-1 µg/mL
UniProt
P08034
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Rat junctional complexes were used as the immunogen for the Connexin 32 antibody. The epitope is known to be located in the cytoplasmic loop (Ref 1) .
Clonality
Monoclonal
Isotype
IgG
Clone
M12.13
Applications
IF, FACS, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Connexin 32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Connexin 32 antibody is available for research use only.
Storage Conditions
Store the Connexin 32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Connexin 32 antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Western blot testing of human stomach tissue with Connexin 32 antibody (clone M12.13) . Predicted molecular weight ~32 kDa.
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