Clathrin Light Chain Antibody
Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A & B) . Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P09496, P09497
Host
Mouse
Reactivity
Human
Immunogen
A recombinant N-terminal protein fragment from human Clathrin light chain was used as the immunogen for the Clathrin Light Chain antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
SPM174
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Clathrin Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Clathrin Light Chain antibody is available for research use only.
Storage Conditions
Store the Clathrin Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Clathrin Light Chain antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Frequently Asked Questions
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