Clathrin Light Chain Antibody

Recognizes proteins of 31-44kDa, which are identified as Clathrin Light Chains (both A & B) . Clathrin is composed of three heavy chains and three light chains, which associate non-covalently to form a triskelion structure. Clathrin light chain regulates the self-assembly of triskelions onto intracellular membranes. Clathrin light chain subunits (LCA and LCB) contribute to regulation of coated vesicle formation to sort proteins during receptor-mediated endocytosis and organelle biogenesis. LCA and LCB are encoded by two discrete genes. They share only 60% homology, and have certain features in common. Both LCA and LCB undergo alternative mRNA splicing, which results in the generation of tissue-specific isoforms.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P09496, P09497

Host

Mouse

Reactivity

Human

Immunogen

A recombinant N-terminal protein fragment from human Clathrin light chain was used as the immunogen for the Clathrin Light Chain antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

SPM174

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Clathrin Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Clathrin Light Chain antibody is available for research use only.

Storage Conditions

Store the Clathrin Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Clathrin Light Chain antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human pancreas with Clathrin Light Chain antibody (clone SPM174) . Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.