FOXA1 Antibody

FOXA1 in breast cancer is highly correlated with ERÎ±+, GATA3+, and PR+ protein expression as well as endocrine signaling. FOXA1 acts as a pioneer factor for ERa in ERÎ±+ breast cancer, and its expression might identify ERÎ±+ cancers that undergo rapid reprogramming of ERa signaling that is associated with poor outcomes and treatment resistance. Conversely, in ERÎ±âˆ’ breast cancer FOXA1 is highly correlated with low-grade morphology and improved disease free survival. FOXA1 is a downstream target of GATA3 in the mammary gland. Expression in ERÎ±âˆ’ cancers may identify a subset of tumors that is responsive to other endocrine therapies such as androgen receptor antagonist treatment. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P55317

Host

Mouse

Reactivity

Human

Immunogen

A human partial recombinant protein corresponding to amino acids 372-472 was used as the immunogen for this FOXA1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

FOXA1/1512

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the FOXA1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This FOXA1 antibody is available for research use only.

Storage Conditions

Store the FOXA1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Optimal dilution of the FOXA1 antibody should be determined by the researcher.

Location

Nuclear

Image Legend

IHC testing of FFPE human prostate carcinoma with FOXA1 antibody (clone FOXA1/1512) . HIER: boil sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.