CD45 Antibody
CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out) . The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P08575
Host
Mouse
Reactivity
Human
Immunogen
Human B lymphocytes were used as the immunogen for the CD45 antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
PTPRC/1461
Applications
FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
CD45 antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.
Limitations
This CD45 antibody is available for research use only.
Storage Conditions
CD45 antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Due to variation in protocol and secondary antibody used, the CD45 antibody may need to be titered for optimal performance.
Location
Cell surface and cytoplasmic
Image Legend
Immunofluorescent staining of PFA-fixed human Jurkat cells with CD45 antibody (clone PTPRC/1461, green) and Reddot nuclear stain (red) .
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