CD3e Antibody
The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. It is required for normal immune responses. This antibody is specific to the epsilon chain of CD3.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL for 60 min at RT, Flow cytometry: 1-2ug/10^6 cells
UniProt
P07766
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 23-119 from human CD3 epsilon chain were used as the immunogen for the CD3e antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
C3e/1308
Applications
WB, FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD3e antibody is available for research use only.
Storage Conditions
Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD3e antibody should be determined by the researcher.
Location
Cell surface and cytoplasmic
Image Legend
Western blot testing of human Jurkat cell lysate with CD3e antibody (clone C3e/1308) . Predicted molecular weight ~23 kDa.
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