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PIP Antibody / Prolactin Inducible Protein / GCDFP-15

It recognizes a protein of 15kDa, identified as Gross cystic disease fluid protein 15 (GCDFP-15), also called Prolactin Inducible Protein, or PIP. It is a major protein component of benign breast gross cysts. It is a known marker of breast cancer, as it is found in approximately 50% of all breast cancer specimens. PIP is a prolactin and androgen controlled protein. This antibody is useful in the identification of metastatic breast carcinoma, or fluid analysis.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P12273

Host

Mouse

Reactivity

Human

Immunogen

A human partial recombinant protein corresponding to amino acids 41-146 was used as the immunogen for the PIP antibody.

Clonality

Monoclonal

Isotype

IgG2a κ

Clone

PIP/1571

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the PIP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This PIP antibody is available for research use only.

Storage Conditions

Store the PIP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the PIP antibody may be required for optimal performance.

Location

Cytoplasmic (Golgi bodies), secreted

Image Legend

IHC testing of FFPE human breast carcinoma with PIP antibody (clone PIP/1571) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20.

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