MART-1 Antibody / Melan-A
MART-1/Melan-A is a protein antigen that is found on the surface of melanocytes. Antibodies against the antigen are used in the medical specialty of anatomic pathology in order to recognize cells of melanocytic differentiation, useful for the diagnosis of a melanoma. The MART-1/Melan-A antigen is specific for the melanocyte lineage, found in normal skin, the retina, and melanocytes, but not in other normal tissues. It is thus useful as a marker for melanocytic tumors (melanomas) with the caveat that it is normally found in benign nevi as well. [Wiki]
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 1-2 µg/mL
UniProt
Q16655
Host
Rabbit
Reactivity
Human
Immunogen
Full length recombinant human MART-1/Melan-A protein was used as the immunogen for this MART-1 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
IHC-P, WB
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the MART-1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This MART-1 antibody is available for research use only.
Storage Conditions
Store the MART-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the recombinant MART-1 antibody for each application should be determined by the researcher.
Location
Cytoplasmic
Image Legend
IHC testing of FFPE human melanoma stained with MART-1 antibody. HIER: steam sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
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