Desmoglein Antibody
Desmoglein 1 (DSG1) is a member of the desmosomal cadherin family. Desmosomes are intercellular adhering junctions that represent cell surface attachment sites for intermediate filament. Desmocollins and desmogleins are the main desmosomal transmembrane proteins. Desmogleins consist of Dsg1, Dsg2, Dsg3, and Dsg4 isoforms. Within the desmosome, the extracellular domain of desmoglein is essential for calcium dependent heterophilic binding to the desmocollins, whereas the intracellular domain is essential for binding to the desmosomal plaque protein, plakoglobin. Desmoglein 1 is synthesized exclusively in the suprabasal layers. Intact and functionally active desmoglein 1 is essential to epidermal integrity.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL
UniProt
Q02413
Host
Mouse
Reactivity
Human
Immunogen
A recombinant protein corresponding to the intracellular domain of human Desmoglein 1 was used as the immunogen for the Desmoglein antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
18D4
Applications
FACS, IF, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Desmoglein antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Desmoglein antibody is available for research use only.
Storage Conditions
Store the Desmoglein antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Desmoglein antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free Desmoglein Antibody (clone 18D4) . Confirmation of Integrity and Purity of the Antibody.
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