Integrin beta 3 Antibody / CD61 / ITGB3

Reacts with human Integrin beta 3, also called CD61. It associates with the Î±V-chain (CD51) to form vitronectin receptor, or with the Î±IIb-chain (CD41) to form the GpIIb/GpIIIa complex (CD41/CD61) . The CD41/CD61 complex appears early in megakaryocyte maturation. The activated CD41/CD61 complex is a receptor for von Willebrand factor, soluble fibrinogen, fibronectin, vitronectin and thrombospondin. It plays a central role in platelet activation and aggregation. The CD51/CD61 is implicated in tumor metastasis and adenoviral infection. The antibody detects platelets in smears of blood and bone marrow, as well as megakaryocytes in frozen sections and cell smears. The antibody is useful for classification of megakaryoblastic leukemia.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (Frozen & FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P05106

Host

Mouse

Reactivity

Human

Immunogen

Human recombinant protein used as the immunogen for the Integrin beta 3 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

ITGB3/1713

Applications

IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Integrin beta 3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Integrin beta 3 antibody is available for research use only.

Storage Conditions

Store the Integrin beta 3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Titration of the Integrin beta 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasmic

Image Legend

IHC testing of FFPE human spleen tissue with Integrin beta 3 antibody (clone ITGB3/1713) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.