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CD28 Antibody

CD28 is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells

UniProt

P10747

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for this CD28 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

C28/1636

Applications

FACS

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the CD28 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This CD28 antibody is available for research use only.

Storage Conditions

Store the CD28 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the antibody to be titered up or down for optimal performance.

Location

Cell surface

Image Legend

SDS-PAGE Analysis of Purified, BSA-Free CD28 Antibody (clone C28/1636) . Confirmation of Integrity and Purity of the Antibody.

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