Beta Catenin Antibody

Beta Catenin is a dual function protein, regulating the coordination of cell-cell adhesion and gene transcription. In humans, the CTNNB1 protein is encoded by the CTNNB1 gene. In Drosophila, the homologous protein is called armadillo. Beta catenin is a subunit of the cadherin protein complex and acts as an intracellular signal transducer in the Wnt signaling pathway. It is a member of the catenin protein family and homologous to gamma-Catenin, also known as plakoglobin. Beta-Catenin is widely expressed in many tissues. In cardiac muscle, it localizes to adherens junctions in intercalated disc structures, which are critical for electrical and mechanical coupling between adjacent cardiomyocyte. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P35222

Host

Mouse

Reactivity

Human, Mouse

Immunogen

A C-terminal 100 amino acid segment from chicken beta-Catenin was used as the immunogen for the Beta Catenin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

5H10

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Beta Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Beta Catenin antibody is available for research use only.

Storage Conditions

Store the Beta Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Beta Catenin antibody to be determined by the researcher.

Location

Cell surface, cytoplasmic, cell junctions

Image Legend

IHC testing of FFPE human tonsil tissue with Beta Catenin antibody (clone 5H10) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.