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Beta Catenin Antibody

Beta Catenin associates with the cytoplasmic portion of E-Cadherin, which is necessary for the function of E-Cadherin as an adhesion molecule.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-3 µg/mL, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P35222

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Chicken recombinant protein was used as the immunogen for the Beta Catenin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

15B8

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Beta Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Beta Catenin antibody is available for research use only.

Storage Conditions

Store the Beta Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Beta Catenin antibody to be determined by the researcher.

Location

Cell surface, cytoplasmic, cell junctions

Image Legend

IHC testing of FFPE human tonsil tissue with Beta Catenin antibody (clone 15B8) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
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