MMP2 Antibody

It recognizes a protein of 72 kDa, which is identified as MMP2. The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, Fibronectin, Laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB) . MMP catalysis requires both calcium and zinc. MMP2 (also designated type IV collagenase) cleaves collagen types IV, V, VII and X and gelatin type I. Activation of MMP2 secretion requires the Ras signaling pathway.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL

UniProt

P08253

Host

Mouse

Reactivity

Human

Immunogen

Amino acids 195-319 were used as the immunogen for the MMP2 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

MMP2/1501

Applications

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the MMP2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This MMP2 antibody is available for research use only.

Storage Conditions

Store the MMP2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the MMP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic, cell surface

Image Legend

Western blot testing of 1) a partial recombinant protein and 2) human U87 cell lysate with MMP2 antibody (clone MMP2/1501) . Expected molecular weight: ~72 kDa (pro form), ~63 kDa (cleaved form) .