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AMACR Antibody (Prostate Cancer Marker)

AMACR, also known as p504S is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

Q9UHK6

Host

Mouse

Reactivity

Human

Immunogen

A recombinant human protein was used as the immunogen for the AMACR antibody.

Clonality

Monoclonal

Isotype

IgG2b λ

Clone

AMACR/1723

Applications

WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the AMACR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This AMACR antibody is available for research use only.

Storage Conditions

Store the AMACR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the AMACR antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

Western blot testing of human PC3 cell lysate with AMACR antibody. Predicted molecular weight ~43 kDa.

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