AMACR Antibody (Prostate Cancer Marker)
AMACR, also known as p504S is an enzyme that is involved in bile acid biosynthesis and beta-oxidation of branched-chain fatty acids. It is expressed in cells of premalignant high-grade prostatic intraepithelial neoplasia (HGPIN) and prostate adenocarcinoma. The majority of the carcinoma cells show a distinct granular cytoplasmic staining reaction. AMACR is present at low or undetectable levels in glandular epithelial cells of normal prostate and benign prostatic hyperplasia. A spotty granular cytoplasmic staining is seen in a few cells of the benign glands.
Product Specifications
CAS Number
9007-83-4
Specifications
Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q9UHK6
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human protein was used as the immunogen for the AMACR antibody.
Clonality
Monoclonal
Isotype
IgG2b λ
Clone
AMACR/1723
Applications
WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the AMACR antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This AMACR antibody is available for research use only.
Storage Conditions
Store the AMACR antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the AMACR antibody should be determined by the researcher.
Location
Cytoplasmic
Image Legend
Western blot testing of human PC3 cell lysate with AMACR antibody. Predicted molecular weight ~43 kDa.
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