Gamma Catenin Antibody / Plakoglobin

Gamma Catenin is a major cytoplasmic component of both desmosomes and adherens junctions, and is the only known constituent common to submembranous plaques in both of these structures, which are located at the intercalated disc (ICD) of cardiomyocytes. It links cadherins to the actin cytoskeleton. Gamma Catenin binds to conserved regions of desmoglein and desmocollin at intracellular catenin-binding sites to assemble desmosomes. [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P14923

Host

Mouse

Reactivity

Human

Immunogen

Recombinant human protein was used as the immunogen for the Gamma Catenin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CTNG/1664

Applications

FACS, IF, WB, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Gamma Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Gamma Catenin antibody is available for research use only.

Storage Conditions

Store the Gamma Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Gamma Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasmic, cell junctions

Image Legend

IHC testing of FFPE human cervical carcinoma with Gamma Catenin antibody (clone CTNG/1483) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.