ASRGL1 Antibody

ASRGL1 (Asparaginase-like protein 1), also known as CRASH, belongs to the Ntn-hydrolase family. Asparaginases utilize asparagine as a substrate to produce aspartic acid and ammonia. ASRGL1 has been identified as a autoantigenic protein that is present in the mid-piece of sperm after obstruction of the male reproductive tract. It is expressed highly in testis, but is also expressed in brain, kidney and gastrointestinal tissues. High levels of ASRGL1 have also been identified in ovarian, uterine and mammary tumors in comparison with normal tissues of the same origin.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

A human partial recombinant protein was used as the immunogen for this ASRGL1 antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

CRASH/1289

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

Store the ASRGL1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ASRGL1 antibody is available for research use only.

Storage Conditions

Store the ASRGL1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the ASRGL1 antibody to be titered up or down for optimal performance.

Location

Cytoplasmic, nuclear

Image Legend

IHC testing of FFPE human endometrium with ASRGL1 antibody (clone CRASH/1289) . Required HIER: steam sections in 10mM Tris with 1mM EDTA, pH9, for 10-20 min.