Alpha 1 Antitrypsin Antibody (Hepatocyte Marker)

Like all serine protease inhibitors, A1AT has a characteristic secondary structure of beta sheets and alpha helices. Mutations in these areas can lead to non-functional proteins that can polymerise and accumulate in the liver (infantile hepatic cirrhosis) . [Wiki]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P01009

Host

Mouse

Reactivity

Human

Immunogen

A human partial recombinant protein was used as the immunogen for this Alpha 1 Antitrypsin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

AAT/1379

Applications

WB, IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Alpha 1 Antitrypsin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Alpha 1 Antitrypsin antibody is available for research use only.

Storage Conditions

Store the Alpha 1 Antitrypsin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Titration of the Alpha 1 Antitrypsin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

Western blot testing of human lung tissue lysate with Alpha 1 Antitrypsin antibody. Expected molecular weight: 47-52 kDa depending on the level of glycosylation.