Alpha 1 Antitrypsin Antibody (Hepatocyte Marker)

The immunohistochemical staining of Alpha 1 Antitrypsin (AAT) is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P01009

Host

Mouse

Reactivity

Human

Immunogen

A human partial recombinant protein was used as the immunogen for this Alpha 1 Antitrypsin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

AAT/1378

Applications

WB, IHC-P

Purity

Protein G affinity

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Alpha 1 Antitrypsin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Alpha 1 Antitrypsin antibody is available for research use only.

Storage Conditions

Store the Alpha 1 Antitrypsin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Alpha 1 Antitrypsin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human tonsil with Alpha 1 Antitrypsin antibody (clone AAT/1378) . Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.