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Calponin Antibody

Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction.

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P51911

Host

Rabbit

Reactivity

Human, Rat

Immunogen

Recombinant human protein was used as the immunogen for the Calponin antibody.

Clonality

Polyclonal

Isotype

IgG

Applications

FACS, IF, IHC-P

Purity

Protein A affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Calponin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Calponin antibody is available for research use only.

Storage Conditions

Store the Calponin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the Calponin antibody should be determined by the researcher.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE rat uterus stained with Calponin antibody. HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.

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