Beta Catenin Antibody

The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs) . AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. [RefSeq]

Product Specifications

CAS Number

9007-83-4

Specifications

Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL

UniProt

P35222

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

A recombinant fusion protein consisting of the N-terminal half of human beta-Catenin fused to maltose binding protein was used as the immunogen for the Beta Catenin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

12F7

Applications

FACS, IF, WB

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1 mg/ml in 1X PBS; BSA free, sodium azide free

Reconstitution

Store the Beta Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Beta Catenin antibody is available for research use only.

Storage Conditions

Store the Beta Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

1 mg/mL in 1X PBS; BSA free, sodium azide free

Applications Notes

Titration of the Beta Catenin antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Location

Cell surface, cytoplasmic, cell junctions

Image Legend

Western blot testing of human 1) A431, 2) A549 and 3) MCF7 cell lysate with Beta Catenin antibody (clone 12F7) . Predicted molecular weight ~85 kDa, but routinely observed at 90-95 kDa.