CD31 Antibody / PECAM-1
CD31 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. It is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [RefSeq]
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P16284
Host
Rabbit
Reactivity
Human
Immunogen
Human protein was used as the immunogen for the CD31 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
FACS, WB, IHC-P
Purity
Protein A affinity
Format
Purified
Buffer
1X PBS, pH 7.4
Reconstitution
Store the CD31 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD31 antibody is available for research use only.
Storage Conditions
Store the CD31 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Titering of the CD31 antibody may be required for optimal performance.
Location
Cell surface and cytoplasmic
Image Legend
IHC staining of FFPE human angiocarcinoma with CD31 antibody. HIER: steam sections in pH7.5-8.5, 1mM EDTA for 10-20 min.
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