ADRP Antibody / Adipophilin / Perilipin 2 / PLIN2

Adipophilin, also called Perilipin 2, ADRP (Adipose differentiation related protein), and PLIN2, belongs to the perilipin family, members of which coat intracellular lipid storage droplets. This protein is associated with the lipid globule surface membrane material and maybe involved in development and maintenance of adipose tissue. It is not restricted to adipocytes as previously thought, but is found in a wide range of cultured cell lines, including fibroblasts, endothelial and epithelial cells, and tissues, such as lactating mammary gland, adrenal cortex, Sertoli and Leydig cells, and hepatocytes in alcoholic liver cirrhosis, suggesting that it may serve as a marker of lipid accumulation in diverse cell types and diseases.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (order BSA/sodium azide-free format for coating), Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 min at RT

Host

Mouse

Reactivity

Human

Immunogen

A human partial recombinant protein was used as the immunogen for this ADRP antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

ADFP/1494

Applications

ELISA, IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

1X PBS, pH 7.4

Reconstitution

The ADRP antibody (with azide) can be stored at 2-8oC. The azide-free format should be aliquoted and stored at -20oC or colder.

Limitations

This ADRP antibody is available for research use only.

Storage Conditions

The ADRP antibody (with azide) can be stored at 2-8°C. The azide-free format should be aliquoted and stored at -20°C or colder.

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Titering of the ADRP antibody may be required for optimal performance.

Location

Cytoplasmic

Image Legend

IHC testing of FFPE human adrenal tissue with ADRP antibody (clone ADFP/1494) . Required HIER: steam sections in pH6, 10mM citrate buffer for 10-20 min.