Plakoglobin Antibody / Gamma Catenin

Plakoglobin, or gamma Catenin, is a common junctional plaque protein. The membrane-associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of Plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE-cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity) . [UniProt]

Product Specifications

CAS Number

9007-83-4

Specifications

Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells

UniProt

P14923

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Recombinant full length chicken gamma Catenin was used as the immunogen for the Plakoglobin antibody.

Clonality

Monoclonal

Isotype

IgG1 k

Clone

15F11

Applications

WB, IHC-P, IF, FACS

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Plakoglobin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Plakoglobin antibody is available for research use only.

Storage Conditions

Store the Plakoglobin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The stated application concentrations are suggested starting amounts. Titration of the Plakoglobin antibody may be required due to differences in protocols and secondary/substrate sensitivity.