CD15 Antibody
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 2-4ug/10^6 cells, Immunofluorescence: 2-4 µg/mL, Immunohistochemistry (FFPE) : 5-10 µg/mL for 30 min at RT
UniProt
P22083
Host
Rabbit
Reactivity
Human, Mouse, Rat
Immunogen
Purified human neutrophils were used as the immunogen for the CD15 antibody.
Clonality
Polyclonal
Isotype
IgG
Applications
FACS, IF, IHC-P
Purity
Protein A affinity
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD15 antibody is available for research use only.
Storage Conditions
Store the CD15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD15 antibody should be determined by the researcher.
Location
Cytoplasmic and cell surface
Image Legend
IHC testing of FFPE human Hodgkin's lymphoma with CD15 antibody. Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min.
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