Alpha Catenin Antibody
Recognizes a protein of 102kDa, identified as Catenin, alpha-1. Catenins comprise a large family of Ca2+-dependent, homotypic cell-cell adhesion molecules that play important roles in development, epithelial cell polarity and tumor progression. Alpha-Catenin is a key regulator of actin dynamics in cell-cell adhesion. During cell-cell adhesion, alpha-Catenin forms a heterodimer with beta-Catenin and links the cadherins to actin associated with the cytoskeleton. Alpha-Catenin also regulates the beta-Catenin signaling in various cells. It displays the tumor suppressor activity and is found to be down regulated in some forms of breast cancer.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 0.5-1 µg/mL
UniProt
P35221
Host
Mouse
Reactivity
Human
Immunogen
A human fusion protein was used as the immunogen for the Alpha Catenin antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
1G5
Applications
FACS, IF, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Alpha Catenin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Alpha Catenin antibody is available for research use only.
Storage Conditions
Store the Alpha Catenin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Alpha Catenin antibody to be determined by the researcher.
Location
Cell surface, cytoplasmic, cell junctions
Image Legend
Western blot testing of human 1) A431 and 2) MCF7 cell lysate with Alpha Catenin antibody (clone 1G5) . Expected molecular weight ~102 kDa.
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