IFN gamma Antibody

Recognizes a protein of 20-25kDa, identified as human interferon. This mAb is specific to human IFNg and recognizes both recombinant and native human IFNg. T lymphocytes and NK cells mainly produce IFNg. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFNa/b (type I interferon) .

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

UniProt

P01579

Host

Mouse

Reactivity

Human

Immunogen

Human recombinant protein was used as the immunogen for the IFN gamma antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

IFNG/466

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the IFN gamma antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This IFN gamma antibody is available for research use only.

Storage Conditions

Store the IFN gamma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

Optimal dilution of the IFN gamma antibody should be determined by the researcher.

Location

Cytoplasmic, membranous, extracellular (secreted)

Image Legend

IHC testing of FFPE human renal cell carcinoma with IFN gamma antibody (clone IFNG/466) . Required HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.