CD15 Antibody
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Product Specifications
CAS Number
9007-83-4
Specifications
Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT
UniProt
P22083
Host
Mouse
Reactivity
Human
Immunogen
The histiocytic cell line U937 was used as the immunogen for this CD15 antibody.
Clonality
Monoclonal
Isotype
IgM k
Clone
Leu-M1 or MMA
Applications
FACS, IF, IHC-P
Purity
Precipitated followed by dialysis in 1X PBS
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD15 antibody is available for research use only.
Storage Conditions
Store the CD15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cell surface and granular paranuclear
Image Legend
Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma stained with CD15 antibody (clone Leu-M1) .
Frequently Asked Questions
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