ACTH Antibody Cocktail / Synacthen

ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This cocktail is specific to Synacthen (aa1-24 of ACTH) ; does not react with CLIP (aa17-39 of ACTH) . POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs) . It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.

Product Specifications

CAS Number

9007-83-4

Specifications

Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT

UniProt

P01189

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Amino acids 1-24 (AH26) and an N-terminal peptide from human ACTH (57) were used as the immunogens for this ACTH antibody cocktail.

Clonality

Monoclonal

Isotype

IgG1

Clone

AH26 + 57

Applications

IHC-P

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the ACTH antibody cocktail at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This ACTH antibody cocktail is available for research use only.

Storage Conditions

Store the ACTH antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The optimal dilution of the ACTH antibody for each application should be determined by the researcher.

Location

Cytoplasm

Image Legend

IHC: Formalin-fixed, paraffin-embedded human pituitary gland stained with ACTH antibody (AH26 + 57) . Required HIER: boil tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.