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Hepatocyte Specific Antigen Antibody

MAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.

Product Specifications

CAS Number

9007-83-4

Specifications

ELISA (For coating, purchase antibody without BSA)

UniProt

Not Known

Host

Mouse

Reactivity

Human

Immunogen

The human hepatocellular carcinoma cell line SK-H1A9-2 was used as the immunogen for this Hepatocyte Specific Antigen antibody.

Clonality

Monoclonal

Isotype

IgG2b κ

Clone

HSA133

Applications

ELISA

Purity

Protein G affinity chromatography

Format

Purified

Buffer

0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

Reconstitution

Store the Hepatocyte Specific Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Limitations

This Hepatocyte Specific Antigen antibody is available for research use only.

Storage Conditions

Store the Hepatocyte Specific Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

Formulation

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

Applications Notes

The optimal dilution of the Hepatocyte Specific Antigen antibody for each application should be determined by the researcher.

Location

Cell surface

Image Legend

SDS-PAGE Analysis of Purified, BSA-Free Hepatocyte Specific Antigen Antibody (clone HSA133) . Confirmation of Integrity and Purity of the Antibody.

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