Hepatocyte Specific Antigen Antibody
MAb HSA133 stains human liver canaliculi and a subset of hepatocellular carcinomas. In frozen sections, it stains liver canaliculi strongly and may be used as a marker of this hepatic substructure. Cell preparations of hepatocellular carcinoma biopsies and cell lines are found to bind this mAb on the cell surface. HSA133 strongly stains liver canaliculi and hepatic carcinoma cells using frozen sections or paraformaldehyde fixed cell preparations.
Product Specifications
CAS Number
9007-83-4
Specifications
ELISA (For coating, purchase antibody without BSA)
UniProt
Not Known
Host
Mouse
Reactivity
Human
Immunogen
The human hepatocellular carcinoma cell line SK-H1A9-2 was used as the immunogen for this Hepatocyte Specific Antigen antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
HSA133
Applications
ELISA
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Hepatocyte Specific Antigen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Hepatocyte Specific Antigen antibody is available for research use only.
Storage Conditions
Store the Hepatocyte Specific Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
The optimal dilution of the Hepatocyte Specific Antigen antibody for each application should be determined by the researcher.
Location
Cell surface
Image Legend
SDS-PAGE Analysis of Purified, BSA-Free Hepatocyte Specific Antigen Antibody (clone HSA133) . Confirmation of Integrity and Purity of the Antibody.
Documents
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