Kappa Light Chain Antibody
This mAb is specific to kappa light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. It recognizes human Ig kappa light chains of both secreted and cell surface immunoglobulin. It detects also free kappa light chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the kappa light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Product Specifications
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT (1), Prediluted IHC only format: incubate for 30 min at RT (2)
UniProt
P01601, P01834
Host
Mouse
Reactivity
Human
Immunogen
Human IgG-kappa myeloma protein was used as the immunogen for this Kappa Light Chain antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
TB28-2
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the Kappa Light Chain antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Kappa Light Chain antibody is available for research use only.
Storage Conditions
Store the Kappa Light Chain antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cell Surface, cytoplasmic and secreted
Image Legend
IHC: Formalin-fixed, paraffin-embedded human tonsil stained with Kappa Light Chain antibody (clone TB28-2) .
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